Microrna
Hello I could give you some protocols If you still need some but I need your email or If you know another way to give you this files.
And could you specify more exactly what kind of information you want then I will give you this what you need.
Hi Everyone, I am working on a research project focused on analyzing behavior and cognition in different environments and situations. I am looking for a solution that allows us to: Record...
29 June 2024 6,540 0 View
Does anyone know an unbiased way to define the cavity and volume of macrocycles, such as crown ethers, cyclodextrin, and similar molecules, using quantum chemistry software? All papers that I'm...
25 March 2024 5,265 8 View
Cities compete with each other in a globalised environment. How does this affect the design of urban space and the community?
04 March 2024 3,319 2 View
Hi, I need to do a co-culture with these two cell types and I would like to do a qPCR. Unfortunately I can't do a flow cytometry so I would use a magnetic beads separation system to isolate the...
19 February 2024 7,155 0 View
I have FTIR spectra and I need to calculate their FWHM. I tried many times on Origin, but never worked. I guess that is something related to the old version that I am using (8.5). Can you help...
09 February 2024 4,241 7 View
I am planning to study a crop grown in lava rocks. What kind of lava rocks is this? I am planning to buy Lava Rocks in online shopping platform. Do you have any idea about the geological features...
21 December 2023 7,238 15 View
I have read about the topic and I was wondering if anybody could help me to interpret the following description of repeated measurements I found in a manuscript (very detailed but still confuse in...
14 December 2023 4,028 3 View
I have bi-weekly UAV flights in soybean trials and I was wondering about removing or not pixels classified as "soil" from the images to calculate veg. indices. Initially, I am calculating for of...
13 November 2023 1,838 1 View
I am calculating the gruneisen parameter for a structure with 28 atoms in a 1x1x1 supercell and 224 in a 2x2x2 supercell, and I am having some issues with phonopy. When I try to run the...
10 October 2023 2,070 2 View
I am running a phylogenetic logistic regression using brm from brms package. My data consists on a categorical predictor (values 0, 1 and 2) and a binary response variabe (values 1 and 2) plus...
06 August 2023 2,998 1 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...
24 June 2024 3,507 2 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...
16 June 2024 9,723 2 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View
The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...
23 May 2024 7,570 3 View
I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...
20 May 2024 5,920 4 View
Hello. Is there any webserver where I can input a set of transcription factors, genes, and miRNAs, and it can provide me with statistical data on how TFs either activate or repress genes and...
18 May 2024 5,848 1 View
Hello all. I have recently been trying to isolate total RNA after transfection of miRNA mimics and antisense inhibitors in mammalian cells. However, the RNA isolated through the trizol method...
13 May 2024 4,935 7 View
would like to work on microRNA to detect colorectal cancer. I will use the type of microRNA type micRNA124,micro143 how I can make primer design for it and how I can choose the primer ?
07 May 2024 8,812 2 View