Hello. Im looking for microRNA protocolos working with PCR in leukemia samples. Thanks !

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They are software's that are used mainly in the discipline of psychology

05 August 2020 9,423 3 View

Which value of volume fraction I can to choose for to identify free surface in an air-water simulation of open channel?

In air-water multiphase flow CFD simulation's it is common to use a VOF = 0.5 to track or identify the position of interface or free surface. I would like to know why this criteria is used and if...

12 July 2020 7,793 9 View

How to decide if to get 1 or various P value? regarding an composting process through time and treatments?

I have a research investigation that I got the results, I applied 4 different composting processes to vegetable waste (manure, manure+Effective Microorganisms, Effective Microorganisms and a...

14 June 2020 2,373 2 View

What is a CFD and other question?

To me, a CFD is solve a fluid mechanics problem using mathematical approaches with the help of a computer. In other words, it is like a marriage between a mathematical model and numerical methods...

04 May 2020 2,811 25 View

Purification of histag protein using HISTRAP HP (GE). What is the best lysis buffer and which protocol may be most suitable?

Purification of histag protein using HISTRAP HP (GE). I am working with insect cells (Tn5B) and my antigens are expressed in the nucleus. I am having difficulty lysing the cells because we have no...

10 March 2020 8,609 2 View

Recent meta-analysis on imaging treatment response predictors in depression?

Dear Experts, Would anyone know of any recent meta-analysis of neuroimaging predictors of treatment response in major depression? I have looked around and all I could find was a paper from 8...

27 January 2020 9,717 1 View

Is there an effect of arbuscular mycorrhizal fungi (AMF) in germination?

Is there an effect of AMF in germination? What I mean can AMF have an ability to infect the hypocotyl, in order to increase the germination percentage, germination rate, and seed vigor? If you...

21 December 2019 9,488 12 View

Any suggestion for the amplification of Wingless in old collection samples?

Hello to everyone, I'm trying to amplify nuclear markers from old museum specimens of beetle (not very old, sampled 10-15 years ago) , I thought on the Wingless fragment, it's obviously a hard...

02 December 2019 7,463 3 View

How to reduce massive protein degradation during purification?

Dear all, I am expressing in BL21(DE3) E. coli cells and subsequently purifying a 6x-His-tagged 50 kDa truncated protein which looks like to be very unstable. Its domains are literally falling...

17 October 2019 1,416 13 View

Why p-values are higher when I run a logistic regression with all variables together, but significant when I do separately?

I ran an experiment with a 2x2 design, like the dependent variable I have a dichotomic variable. When I running a factorial ANOVA, interaction is significant and one principal effect, also when I...

14 October 2019 9,342 6 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View

Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Can I predict miRNA by knowing the mRNA target?

Hello! I am trying to predict in silico the miRNA by knowing the target. Most of published research starts by knowing the miRNA and predicts the target using online tools. Are you familiar with...

31 December 2020 7,433 5 View

What are the possible drawbacks of using miRNA sponge to enhance the specificity of gene expression?

I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...

26 December 2020 9,921 2 View