Hello, I have individual DNTP-DATP,.... can anyone help me about the protocol for the RT-PCR reaction mixture for microRNA?

More Razwoan Bakth's questions See All

Hi, how can I isolate microRNA from platelets?

Platelets, microRNA

01 January 1970 1,290 2 View

Hi, Can any one tell me what is the good con. for RNA from plasma and serum isolation. my RNA con. less then 10 ng/microliter?

RNA, Plasma, Serum, Isolation, concentration.

01 January 1970 7,470 4 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

How can I add a new WSN routing protocol in NS2 or NS3?

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19 February 2021 7,981 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

I want to simulate a privacy-preserving algorithm in MQTT for IoT devices, but I'm not sure which simulation software is best suited for this?

I want to apply a privacy-preserving algorithm in MQTT for IoT devices. Which tool do you suggest to simulate this algorithm? I want to measure the performance on the IoT battery device? Actually,...

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Christopher Dacosta

Hi Razwoan,

For measuring microRNA expression by RT-PCR, you would probably want to use stem-loop primers for the reverse transcription (RT) step (see https://www.ncbi.nlm.nih.gov/pubmed/21732315). If you wish to do TaqMan real-time RT-PCR and have the appropriate equipment, I have personally used the TaqMan Real-Time PCR Assays (http://www.thermofisher.com/uk/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays.html).

Good luck!

Chris