Hi Razwoan,

I think 10ng/uL is sufficient from the serum samples, requirement might be high for downstream experiments but you can't get much more that you got from serum samples but you can pull accordingly. 

 Thanks

Hello Razwoan

Have you tried with other extraction kit for getting a better performance?

for example:

http://www.mn-net.com/tabid/1324/default.aspx

http://www.mn-net.com/Portals/8/attachments/Redakteure_Bio/Flyer/FL_NSmiRNAPlasma.pdf

Regards

I have used both exiqon and qiogen. Thanks  I will try them