Hello, I have antibiotic disks of concentration 30 μg each. I need to prepare 1mg/mL solution. How do I proceed ?

More Naz Mnb's questions See All

Maximum Likelihood Estimation of Parameters issue ?

I have data set consist of x and y variable where I wan to perform maximum likelihood estimation (MLE) to fit a mean function and astandard deviation function o the data. I am estimating the the...

21 April 2024 1,734 7 View

How far medical science reached in the treatment of diabetes type 2 ?

Is it possible to cure diabetes clinically? There are huge number of diabetes patients around the world. what are the main causes of this medical conditionand what about history of diabetes. when...

01 March 2024 6,084 7 View

Can we consider incident rate ratio equal to hazard ratio in meta-analysis?

In a meta-analysis, there is just one study that reported the incident rate ratio, can I consider it as HR?

03 November 2023 1,845 4 View

How can i deposit some metal particles on TiNts sheets using electroless deposition , so that there is reduction occuring only on the TiO2 Sheet?

I have a problem with it, as the whole solution changes colour and the reduction occurs in the whole solution. I just want that the particles get deposit on the Ti naotubes grown on Sheet.

20 October 2023 9,576 2 View

If Y shows variations with X which technique shall i apply to catch patterns in it ?

The dataset I am working with consists of two columns, X and Y where the function Y changes for increasing values of the X variable. If Y shows variations with X, the simplest way to do this is...

21 August 2023 1,088 10 View

How to justify high RMSD (it is high but stable)?

I conducted a 100ns simulation of my docked complex, obtaining a maximum RMSD of 4.2 Angstroms and an average of 3.1 Angstroms. Can someone help me how can I justify this high RMSD? I have...

30 July 2023 4,291 13 View

How to take a permission of Academic Stress scale by kim 1970?

hello! I am Momina Naz, from Capital University Of Science and technology. I need permission for Academic Stress Scale by kim 1970 For my research purpose but unfortunately i did’nt find author’s...

25 July 2023 9,335 0 View

Transfection protocol to transfect A549 CELLS ?

Anyone who can help me in transfection of A549 cells. I have done several times but it doesn't work properly. Thank you so much.

16 July 2023 10,010 5 View

Inquiry about Medium and Conditions to Induce Phenazine Production in Pseudomonas ?

Medium Composition: Could you kindly suggest a medium formulation that has proven effective in inducing phenazine production? Please include the specific carbon and nitrogen sources, inorganic...

25 June 2023 3,191 1 View

Protocol to label the membrane with N-(lissamine rhodamine B sulfonyl)-phosphatidylethanolamine (N-Rh-PE)?

Hi everyone Can anyone will describe the best protocol to label the membrane with N-(lissamine rhodamine B sulfonyl)-phosphatidylethanolamine (N-Rh-PE). I have to do in cardiac cells(primary and...

22 June 2023 790 0 View

How to calculate FIC index?

Can someone explain to me how was the MIC of MO in combination and MIC of antibiotic in combination was calculated? as shown in the attached file below.

26 July 2024 2,702 1 View

Are those seeking solution part of the problem?

In reference to antibiotic as a commodity, what is your take and way forward on common molecules in both Veterinary and Human antibiotics in relation to currently experienced emergency of AMR?

20 July 2024 4,020 0 View

Does it work as antibiotic resistance if plamid has no RBS??

I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...

11 July 2024 7,847 1 View

Is antibiotic bad for transfection?

I'm going to transfect cells with expression plasmids and later siRNAs against the same gene. Is it true that penicillin/streptomycin in the media negatively affects these processes? Shout I use...

07 July 2024 8,671 6 View

Stable transfection be combined with two or more antibiotics to screen cells?

Can positive cell screening after stable transfection be combined with two or more antibiotics to screen cells? Will all the cells die?

10 June 2024 9,739 0 View

I want to ask if I suppose to add 200mg of antibiotic in 100ml of water so what is the concentration of this stock solution in microgram?

Hello, I recently started working with antibiotics and I do not have that much experience of making dilutions accurately. I want to ask if I suppose to add 200mg of antibiotic in 100ml of water...

08 June 2024 2,285 6 View

Is it okay to dissolve Ampicillin Anhydrous in 1 M NaOH?

So I made a 100 mg/ml solution of Ampicillin and in order to solubilize the powder I used 1 M NaOH. I noticed the solution turns yellow shortly after dissolving and I was wondering if some...

05 June 2024 7,170 0 View

Why am I observing a m/z peak at 372 for the ciprofloxacin hydrochloride hydrate spectra in MS?

The antibiotic powder is dissolved in Milli-q water and then filtered using syringe filter for the analysis.

01 June 2024 7,419 0 View

How Can I Understand the Different Results from MacConkey Agar and β-Galactosidase Assays in a LexA-Based Bacterial-Two Hybrid System?

I'm conducting protein-protein interaction studies using a LexA-based Bacterial-Two Hybrid system with E. coli reporter strains. We employ MacConkey agar and β-galactosidase assays to evaluate the...

26 May 2024 4,647 0 View

Primocin shelf life at 20degC?

Hi all, I am wondering if anyone has any experience with using InvivoGen's Primocin antibiotic solution beyond the indicated expiry date when properly aliquoted and stored at -20degC. We have a...

20 May 2024 7,613 2 View