What are the political factors affecting curriculum design at the primary level in Pakistan?

This Topic I chose for my Thesis in Final Year of my Master's. I am in process of it's completion but need more suggestions and comments for the Topic to elaborate.

14 December 2020 356 3 View

What is the optimal dose of tamoxifen by oral gavage in adult mice?

I am using a Cre-ERT mouse model and giving mice tamoxifen by oral gavage at 8 weeks of age. 1 dose of 6 mg of tamoxifen does not generate enough Cre activity. Does anyone have any recommendations...

17 October 2020 5,034 2 View

What should be the dimension of the composite specimens which has 10 mm thickness according to ASTM Standards?

I have carbon fiber composite specimens and wonder about the dimensions of the specimens which are 25 mm wide, 250 mm long and 10 mm thickness is compatible with the ASTM D3039 Tensile test...

08 October 2020 1,261 4 View

Optimum conditions for Cell cycle analysis via flow cytometry?

I want to ask about the RNAse treatment for the cell cycle. What should be the ideal conditions during RNAse treatment i.e. Incubation period, temperature, time or either it should be placed in 5%...

15 March 2020 2,227 5 View

AMD in NAMD or flooding in GROMACS?

Which software would you recommend for aMD? Gromacs or NAMD? Also, what do you think about Gaussian aMD implementation in NAMD? Is it preferable to others?

29 October 2019 2,981 2 View

Whay band gap of MoS2 reduced after TM doping?

I have doped Cr, Mn, Co, Ni and Cu in bilayer MoS2 but the band gap reduces after Cr and Mn doping, and other three elements (Co, Ni and Cu) convert the semiconductor into metal. can anybody tell...

08 July 2019 3,025 4 View

Can anybody provide protocol for the lipase inhibition assay using olive oil ?

I am using lipase of aspergillus niger for lipase inhibiton assay against some molecules. using substrate olive oil. Kindly if anybody have protocol? Thanks

12 February 2018 3,962 3 View

Is we use same slides for scanning electron microscope that has been used for histology???? if yes than either it needs any processing if yes how?????

I have prepared slides for histological studies. I want to do SEM for that specimens. Can same slides would be used after some treatments???? If yes what kind of treatment should I use for this....

19 November 2017 1,598 3 View

I am doing MD simulation of membrane protein by Gromacs at add ions step it give fetal error that atom LC3 does not found.what shoud i do?

MD simulation of membrane protein

06 October 2017 5,253 2 View

Is there any antibiotic that is effective only against gram positive cocci but not against gram positive bacilli?

I am facing continuous problem of gram positive cocci contamination in my media. I want to grow gram positive bacillus. Yes, I am aware of other microbiological techniques, but is the any...

30 June 2017 7,765 6 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

What are the naitve functions of bacterial NRP?

The bacterial NRPs (nonribosomal polypeptides) are famous as clinical drugs such as antibiotics, but is there other native function of these NRPs of bacteria except antimicrobial, because their...

01 February 2021 4,280 1 View

Why I am not getting cultures of DH5 alpha and BL21 cells after transformation?

Even if I get them cultured colonies are very less and almost not transferable and grows very slowly in the media. I checked cells by transforming with control vector. I changed antibiotic also...

06 January 2021 1,013 3 View

Is it normal to use Tris buffer when testing for bacteriostatic properties of isolated compounds?

Hello, to start off I attempting to test the bacteriostatic properties of certain compounds. I bought a kit that contains some of the equipment to perform this analysis, however, in the included...

02 January 2021 2,338 2 View

Is there any database or website from where I can get a list of FDA approved drugs for treatment of certain urinary tract infection?

I want to know all prescription drugs which are FDA approved compounds for infection like Urinary Tract Infections along with their causative agents. Can you guide me about any resource, database...

17 December 2020 4,852 3 View

Does anyone experience E coli lysis while seed culture?

Hi, I am currently working with BL21(DE3)PLySs and PRSET A vector. Out lab frequently transformation and culture E Coli for protein purification. But recently, even though there is colony after...

13 December 2020 9,167 9 View

How can i get nonsporulating bacterial bacillus subtillis strain spoIIE without antibiotic growth conditions ?

10 November 2020 7,168 4 View

Where can I find a database of antibiotics-dose required to kill bacteria?

Hi, I am Zaizhi. I would like to know where I can find a database of antibiotics-dose required to kill different bacteria. I found a few years ago. There, I was able to know the range of dose of...

08 November 2020 9,276 3 View

Which dose do you expose RAG1 deficient mice for lethal-irradiation?

Hello, I'd like to apologize for my English in advance, I'm still trying to polish it. We are performing several bone marrow transplantations (BMT) or fetal liver transplantations (FTL) to...

05 November 2020 4,239 1 View