I am testing protein-protein interaction. I did 5 repeats of the chosen colonies, but I am not sure if its okey. What is the minimum repeat number?
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Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...
02 February 2021 3,861 3 View
Hello, I'm working on primers from the publication: Okodo M, Okayama K, Teruya K, Sasagawa T. Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types. J Med Virol....
24 January 2021 7,636 3 View
I am looking for a way to prepare a mounted bio sample for testing superresolution methods, such as fixed cell culture. So far, most protocols I found for Alexa647 and other dyes require liquid...
12 January 2021 6,040 3 View
04 January 2021 3,293 1 View
I have conducted a systematic review and one of the reviewers had mentioned about missing out on some good studies from the sample. on the surface the study is on a different topic but after an...
21 December 2020 573 4 View
Hi, I've determined the glucose content in Cellic CTec2 enzyme blend and it is 12-14 mg per 100 mg of enzyme blend. Could anyone share with me your results so I can compare it with mine?
08 December 2020 6,312 3 View
I had a few students help me with a simple but time-consuming task. The data they helped with will be used in a scientific paper. The students are part of the Student Research Program (SRP) at my...
11 October 2020 4,514 12 View
Hi there, I am an undergraduate student in the UK and started to work on my dissertation, my subject is around common household dust. However, I can't seem to find any articles on basic and...
08 October 2020 6,838 1 View
I did find some papers that incorporate the two ideas together but I could not follow the research methodology. The simulation results were remarkable but not self explanatory. I tried to follow...
20 September 2020 5,354 4 View
Dear all,, I am working on fatty acid chloride. So I wanted to find out the molecular weight of fatty acid chloride? So it is possible to find out using GPC or GCMS or there is any tritiometric...
06 September 2020 5,139 6 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
02 March 2021 3,060 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
What do you know about biotechnology art?
02 March 2021 1,653 3 View