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There are several methods for assessment of antioxidant activity, of which DPPH scavenging assay. DPPH has absorbance around 520nm, while carotenoids have absorbance near 500nm.
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Using DPPH antioxidant microtiter plate assay for the AgNPs produced by Klebsiella species; I get Absorbance greater than that of the control meaning that AgNPs has negative antioxidant activity...
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