Hello, how can i find a primer to identify Bacillus Sp. with PCR? Can someone help me with this?

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Karen A. Darbinyan

Hello, use

B27f (5'-AGAGTTTGATCCTGGCTCAG-3') and

U1492R (5'- GGTTACCTTGTTACGACTT-3')

as universal bacterial primer for positive amplification approval and

use below bacillus specific primer

463 F (5’CTAAAACTCAAAGGAATTGACG3’) and 463R (5’AATACGTTCCCGGGCCTT3’).

for bacilli detection.

Good luck!