Hello everyone. Could you suggest some applicable tools to simulate a PCR reaction with determined primers ?

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Katie A S Burnette

Do you mean a "primer BLAST" to see if your primers are specific? Easy to do in NCBI BLAST.

Pushkal Sinduvadi Ramesh

You can simulate most of the molecular biology experiments using tools like Snapgene or Geneious Prime. You can get trial versions for about a month.

Best!

Fredrick Odoyo Ondiek

Hossein Ghahramanpour How about Primer3, NCBI's Primer-BLAST, and Geneious that allow simulation of PCR reactions by inputting specific primer sequences, aiding in determining amplicon size and optimizing PCR conditions?