Hello Everyone , can anyone suggest me how to calculate dendritic spine numbers using image J or any other software?

More Prema Singaravel's questions See All

HeLa cells have been detaching after heat stress. Can someone please suggest some way to keep the cells adhered during heat stress and recovery?

I have been growing HeLa to 90-100% confluency in 12 well dish, prior to subjecting them to heat stress at 43 degrees Celsius for 1.5hrs in a water bath. Unlike HEK293T, the HeLa cells lose...

16 April 2024 7,328 0 View

How to extract intense rainfalls (e.g., annual maximum rainfall for 24-hr duration) from the historical records of daily rainfalls?

Generally, the daily rainfalls are recorded at fixed time intervals (e.g., from 9 am to 9 am). We can find such records of historical rainfalls for centuries. However, in such records, even the...

20 November 2023 5,759 2 View

Hi, can anyone suggest anti-ubiquitin antibody that is specific for mono-ubiquitination and does detect not poly-ubiquitination or free Ubiquitin?

In our in-vivo assays, we see a modified band which we know is a mono-ubiquitin band. We still want to confirm with a mono-ubiquitin specific antibody, if the same is available commercially.

19 November 2023 7,374 0 View

What are the criteria to be adopted in sizing the gates of salinity barriers constructed across the rivers drain flood prone areas?

I have a problem of designing a salinity barrier on a river flowing through a large agricultural area, susceptible for flooding, to prevent saltwater intrusion to domestic water supply intakes....

02 November 2023 2,087 0 View

What is the real cause behind the failure of ‘Yala’ (2023) cultivation under the 'Udawalawa' Scheme? Drought, wrong decision or some other?

This question is mainly targeted to Sri Lankan Engineers, Meteorologists, Agronomists and Lawyers. Even the foreign researchers and consultants who familiar with the system (‘Udawalawa’ &...

06 September 2023 8,987 0 View

What are the adverse effects of using freshwater reservoirs for generating solar energy?

Mine is a tropical country which has developed freshwater reservoirs in different eras of the history (new and ancient), mainly for the purpose of irrigation and hydropower. Those reservoirs are...

26 August 2023 5,463 1 View

What are the laws and regulations available for securing riparian rights of water in river systems and priority order of water using activities?

Udawalawa is a large reservoir constructed in 1960s, mainly for the purpose of irrigation. Later, around 1990s, hydropower reservoir was constructed at the upstream of the same catchment...

11 August 2023 3,799 2 View

I am shocked to see that the co-author of my journal article has been changed?

How is this possible on ResearchGate?????

20 July 2023 4,448 3 View

The person s.Sundar has included his name as a co-author in my paper. How should I remove him?

He is irrelevant to my research area.

30 March 2023 2,611 2 View

The Stereotaxic coordinates?

CAN ANYONE HELP ME WITH THE STEREOTAXIC COORDINATES FOR SOMATOSENSORY BARREL CORTEX OF P5,P7 & P14 MICE.

24 July 2022 9,152 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

Dirty and clean?

Hi everyone I need a file with a dirty and clean potato image

04 August 2024 7,199 4 View

Hello, regarding Mxene 2D titanium carbide?

I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.

02 August 2024 6,789 4 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Why in 2D image created by discovery studio 2021, the halogens appear as alkyl group?

In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.

28 July 2024 734 0 View

How to extract RNA from neurons?

Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...

23 July 2024 6,783 1 View

Why is there a significant edge deviation in radar point cloud and camera registration?

The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...

23 July 2024 7,479 3 View

How do you get pure primary macrophage cell culture?

I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...

23 July 2024 4,074 2 View

Michael N. Cranston

If you have access to Neurolucida 360, this can be done automatically through their automated processing.

Prema Singaravel

Thank you for your response. No, i do not have that software. i am using only imagej. will that suffice?. one more question should i use whole neuron to count the apical dendrites or only the portion of dendrite would be fine?.