CAN ANYONE HELP ME WITH THE STEREOTAXIC COORDINATES FOR SOMATOSENSORY BARREL CORTEX OF P5,P7 & P14 MICE.
I have been growing HeLa to 90-100% confluency in 12 well dish, prior to subjecting them to heat stress at 43 degrees Celsius for 1.5hrs in a water bath. Unlike HEK293T, the HeLa cells lose...
16 April 2024 7,328 0 View
Generally, the daily rainfalls are recorded at fixed time intervals (e.g., from 9 am to 9 am). We can find such records of historical rainfalls for centuries. However, in such records, even the...
20 November 2023 5,759 2 View
In our in-vivo assays, we see a modified band which we know is a mono-ubiquitin band. We still want to confirm with a mono-ubiquitin specific antibody, if the same is available commercially.
19 November 2023 7,374 0 View
I have a problem of designing a salinity barrier on a river flowing through a large agricultural area, susceptible for flooding, to prevent saltwater intrusion to domestic water supply intakes....
02 November 2023 2,087 0 View
This question is mainly targeted to Sri Lankan Engineers, Meteorologists, Agronomists and Lawyers. Even the foreign researchers and consultants who familiar with the system (‘Udawalawa’ &...
06 September 2023 8,987 0 View
Mine is a tropical country which has developed freshwater reservoirs in different eras of the history (new and ancient), mainly for the purpose of irrigation and hydropower. Those reservoirs are...
26 August 2023 5,463 1 View
Udawalawa is a large reservoir constructed in 1960s, mainly for the purpose of irrigation. Later, around 1990s, hydropower reservoir was constructed at the upstream of the same catchment...
11 August 2023 3,799 2 View
Kindly give suggestions regarding calculation of dendritic spine density .I have a doubt that whether i have to calculate the spine numbers directly under microscope or from acquired images . To...
24 July 2023 559 2 View
How is this possible on ResearchGate?????
20 July 2023 4,448 3 View
He is irrelevant to my research area.
30 March 2023 2,611 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...
31 July 2024 9,859 0 View