ali bristit janakari chaiyo k

Dear Mr. Pathak.

As I experienced for sample preparation, the manufacturer's protocol will explain it. 

Generally :

1. Collect the blood in heparized tube.

2. Then you should decide whether you want the serum or the plasma. Using cenrifugation, separate the plasma or serum, I used 6000 rcf for 4 minutes.

3. Take the serum or the plasma then process as the kit's instruction.

4. If you need to store the sample, keep in -20 degree for short term storage or -80 degree for long time storage.

For example of ELISA kit intruction

Hope it hepls

http://www.eiaab.com/UploadFiles//20109122543979729.pdf