I currently study about the Stroma of pancreatic ductal adenocarcinoma. Therefore I had to deal with various Extra cellular matrix stainings using both IHC and chemical staining methods. Sometimes, it does not help me when I analyze them by “area measurement tool from ImageJ” because there is no difference by means of area, rather there is clear difference in the intensity of the staining between my two different genotypes.
It would be greatly helpful if someone could suggest me a method to do the above.
Thank you in advance!
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