Can anyone let me know where I can get the Cycloviolacin O2 (chemically synthesized) ?

Cycloviolacin O2 is a cyclic peptide that has three disulphide bonds and reported for various biological activities. I am looking for the sources where I can get the chemically synthesized...

21 November 2019 423 3 View

Other than 1,8-naphthalimide dyes, is there any other molecules which can absorb two-photons at a time?

Other than 1,8-naphthalimide dyes, is there any other molecules which can absorb two-photons at a time with possible functional groups which allows modifications

21 May 2018 7,499 8 View

What is the precision of Matlab?

By observing in articles belong to Meta-heuristic algorithms, the best and mean solutions are like 10^{-150} or 10^{-200}. Are they right. What I observed, Matlab considers 10^{-324} equal to...

19 April 2018 8,267 4 View

Hello, Does someone know how to evaluate the intensity of DAB in IHC and the intensity of other chemical staining?

I currently study about the Stroma of pancreatic ductal adenocarcinoma. Therefore I had to deal with various Extra cellular matrix stainings using both IHC and chemical staining methods....

14 February 2018 8,755 1 View

Is it possible to observe protein aggregation using UV - Vis spectrophotometer?

02 April 2017 2,665 8 View

What is the best method to purify basic proteins with pi 7.8?

13 April 2015 5,256 5 View

How can I increase the binding affinity of GST-tagged protein to GST sepharose column?

01 March 2015 7,678 4 View

Why is my protein getting precipitated, even at low concentrations?

I am purifying a recombinant protein (host Rosetta) with his tag. I am getting reasonably good yield (4-5 mg/liter of culture). However after affinity purification using Ni-NTA column, during...

19 June 2014 9,136 4 View

Can anyone help me figure out why I am losing protein during ion exchange chromatography?

I am purifying a protein from a bacterial system and I was able to successfully purify the protein through an affinity column. My protein has a pI of 5.9 and it is stable at higher pH. After...

01 June 2014 8,858 5 View

How much of Dnase can be added and what buffer can be used for cell lysis during protein extraction?

I am isolating protein from more volume of culture. However I am unaware how much dnase to use and also what buffer to use for its activity, if I am dissolving the pellet in 30ml of lysis buffer...

02 July 2013 7,943 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View