i need a protocol for transfecting the oocyte with FAM labelled LNA Blockers using Lipofectamine RNAi MAX as a transfecting agent. i use to pool 10 oocytes in 100ul of transfecting solution. kept the oligo concentration upto 50nM and at various lipofectamine (1,2,4,8ul), with several incubation periods ( 45 mins, 90mins, 180, mins). Please suggest me any solution on any variable such as incubation time, lipofectamine concentration, or oligo concentration. Many Thanks
Very interesting topic actually!
I am wondering why not using a polymer-based transfectant if there are potential issues with lipd-based reagents (for the protocol proposed by Luìs).
If you both want to try our Viromer BLUE, we would be very grateful to learn about its potential for transfecting oocytes. Please, tell me, I can provide a free sample.
Bests, Sandra
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