Hi, I have mice expressing Cre under the Nestin promoter and the floxed gene. where should I expect deletion of the gene? In the whole brain? only neurons or also glia?
As far as I know, there are no whole brain inducible lines. However, you can cross mice to Nestin-Cre to perform whole brain deletion or to CamK2a-Cre to perform forebrain (starting from postnatal day 14) deletion of your target gene. Unfortunately, the Nestin-CreER will only target dividing cells and therefore its domain will be restricted to the adult neurogenic zones in the mouse.
Thanks, Maxime. Where in the brain should I expect my floxed gene to be deleted? Everywhere, or only in regions that are rich in neurons (as opposed to glia)?