14 Questions 10 Answers 0 Followers
Questions related from Iddo Magen
I have Trizol LS that was manufactured in 2017 and was stored at RT.
16 May 2023 8,036 3 View
Hello I accidently forgot to remove traces of 70% ethanol used to wash beads, and did the elution for my libraries with water right away. So now my libraries contain a bit of ethanol, I do not...
13 January 2022 4,792 1 View
Hello, Does anyone know about cheap services for library preparation of small RNAs from biofluids? It may also include RNA extraction/sequencing and can be outside North America/Europe. Thanks
31 August 2021 6,432 3 View
Hi I am going to slice paraffin embedded spinal cord. I have two questions: 1. How do I put the SC in the paraffin block so that it will be in coronal orientation? It is not trivial as the part...
14 August 2020 7,695 2 View
I am going to do in situ hybridization for adult mouse brain and spinal cord. I will post-fox the tissues in 4% PFA ON and then embed in paraffin. Should I also perfuse the mice before with PFA? I...
11 August 2020 4,152 6 View
Hi I wish to perform ddPCR for miRNAs in blood plasma. I have a kit for preparing cDNA with a universal RT primer that uses poly-A tail as a template, and a Taqman probe for qPCR that is called...
14 June 2020 3,625 3 View
Hi , i performed multivariate Cox regression analysis and one of the covariates had a p-value of 0.08. since it is a replication study and i have a hypothesis, I can have a directional...
03 May 2019 7,494 2 View
Hi everyone, I did RNA-seq for small RNA, and now I want to confirm the results with qPCR. What is the best way to find reference miRNA genes from the NGS data, for the normalization of the...
13 January 2019 1,646 3 View
Hi, i performed NGS for small RNA and ran DESeq on the data. there is one miRNA that is upregulated by 2-fold in the patients compared to healthy controls. However, when i look at the raw...
05 July 2018 6,281 3 View
Hi, I need to isolate plasma from blood for RNA extraction afterwards. Does it have to be in a cold centrifuge? Do you need in general to isolate plasma in a refirigerated centrifuge or is it...
27 May 2018 1,401 4 View
Hi, I got these results in tape station (attached). The small RNA library size should be around 173bp, so I think that libraries 28, 31, 34, 36, 37 also have an adapter dimer. I am going to...
02 October 2017 554 1 View
Hi, I would like to extract RNA from biofluids, and we have a home-made protocol that I got from a collaborator, that uses Zymo spin columns. However, we have bunches of miRNeasy micro minelute...
14 June 2017 8,427 1 View
Hello, If I use a PCR plate for library prep and I need the products after each step in the protocol (in my case it's polyadenylation, RT and PCR), is it safe to open the plastic foil that covers...
26 April 2017 8,558 3 View
Hello, Regarding reliability of RNA-seq data - I sequenced a serum sample with the NEB kit and a new kit from Clontech that is PCR-based and not ligation-based so it is supposed to introduce less...
22 April 2017 4,090 5 View
