Hello, can anyone provide me with a simple method to extract proteins from bacterial cell cultures?

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Statistics(and tests) and publications-clarification?

It is well known of the importance of statistics in research to support your answers. Please define any statistical test, what does it do and where to apply them. Please explain the difference...

28 May 2021 3,268 9 View

Basic information on taxonomy?

Sometimes abbreviations are more common than we ever thought. We can at least say some abbreviations are more common than others, and they appear frequently on literature. However, sometimes, the...

18 May 2021 5,941 26 View

How to perform AIC Model Selection for A Binary PGLMM?

I'm trying to do a model selection for a PGLMM for binary data. I have used AIC before for glm using this code (MuMIn package) df_dredge

03 May 2021 9,713 3 View

Nomograph-is it an obsolete method?

I understand there are conversions between the gs on a system centrifugation and if not mistaken when it is from an automated process to a simple "plate -keep the system balanced" (Please correct...

28 April 2021 2,894 0 View

Matrix of MALDI-TOFF. How to choose?

When you se parate the ions in m/z in MALDI-TOFF you have an absorption-ionization matrix of a material of agarose. How does this selection affect the Time of flight, why would you pick this...

03 March 2021 3,812 4 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,669 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,723 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 4,334 4 View

Hello, does anyone know about a free available webtool to calculate plant root area from 2D pictures in jpg format?

Pictures are from clean banana roots of plants grown in greenhouse for 9 or 15 weeks

07 June 2020 8,883 9 View

Are you Interest in joint research?

Regards I am a research professor at the Universidad Cooperativa de Colombia www.ucc.edu.co and the Free University www.unilibrecali.edu.co in the city of Cali in Colombia, South America. I am...

09 October 2018 4,641 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Help on understanding the implementation of Mori Tanaka method on MATLAB?

I am new to Micromechanics and having similar problem with understanding the implementation of the formula's. I would appreciate if anyone can guide me on how to go about getting a scalar value...

30 July 2024 969 0 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

How to interpret results of AST for Pseudomonas aeruginosa?

While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...

25 July 2024 9,229 1 View

Farhana Rumzum Bhuiyan

https://www.sciencedirect.com/science/article/pii/S2405844018308703

https://doi.org/10.1016/j.heliyon.2018.e00646

Alex Ignatov

Jassmine Zorrilla Look at Motta, Sara, Davide Vecchietti, Alessandra M. Martorana, Pietro Brunetti, Giovanni Bertoni, Alessandra Polissi, Pierluigi Mauri, and Dario Di Silvestre. 2020. "The Landscape of Pseudomonas aeruginosa Membrane-Associated Proteins" Cells 9, no. 11: 2421. https://doi.org/10.3390/cells9112421

Jassmine Zorrilla

Thank you very much Alex Ignatov , this is a useful paper.