Total monomeric anthocyanin content (mg/L of fresh weight)=(Abs*MW*DF*1000)/MA*PL

Here,

MW=449.2 g.mol−1 for cyanidin-3-glucoside (molecular weight)

DF-Dilution factor

Molecular absorptivity= 26,900 M

PL=Path length (cm)

Reference - Wrolstad and Giusti (2001)

You can find the TMAC value in dry weight by subtracting the moisture content from 100. Find TMAC that value using above value.

DW=100-Moisture content

TMAC (g/100g in DW)=TMAC (Value from wrolstad formula) *100/Dry weight

Hope it helps

Best wishes

Hola

1. Calcular la absorbancia de la muestra diluida (A) de la siguiente manera:

A = (Aλ vis-max - A700) pH 1,0- (Aλ vis-max - A700) pH 4,5

A = (1.385 – 0.024) - (0.191 – 0.010)

A = 1.18

2. Calcular la concentración de pigmento antociánico monomérico en la muestra original mediante la siguiente fórmula:

Antocianina monomérica (mg/L) = (A × MW × DF × 1000) / (ε × 1)

Donde: MW es el peso molecular (Tabla F1.2.2), DF es el factor de dilución, y ε es la absortividad molar (Tabla F1.2.1).

Antocianina monomérica (mg/L) = (1.18 × 449.2 × (25/5) × 1000) / (26900 × 1)

Antocianina monomérica (mg/L) = 98.52

Convertir a mg/g:

Antocianina monomérica (mg/g) = ((98.52 x 200) / 1000) /20

mg/g = 0.99

Convertir a % en base húmeda:

Antocianina monomérica (% bh) = (0.99x100) /1000

%bh = 0.10

Convertir a % en base seca:

Antocianina monomérica (% bs) = (0.10/ (100-87.47)) x 100

% bs= 0.80

Saludos!!!