Have you examined this using multiple cell population recording? Have you examined the drug effects on the different subtypes of delay neurons?

19 April 2014 0 5K Report

Very interesting and relevant work on the alpha 7 nicotinic receptor, I'm curious to know if you have further examined the cellular and molecular basis of the alpha 7 nAChR role in cognition / attentional processing / WM / cell functional tuning?

Article Nicotinic 7 receptors enhance NMDA cognitive circuits in dor...

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Science method

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Scholarly Journals Accepting Submissions?

Could you share other peer-reviewed journals that have accepted your scholarship? I am seeking to publish a new article focused on diversity and student engagement or write a book chapter. Any...

19 June 2024 4,716 0 View

Correct a reference ?

Hello, There is an error concerning the publication https://www.researchgate.net/publication/369560828_Non_recours_aux_services_d'accueil_temps_libre_et_aux_milieux_d'accueil_de la...

28 April 2024 5,533 2 View

How to promote Aboriginal and Torres Strait Islander teaching to non-indigenous students in an authentic manner?

Early Childhood Primary

08 October 2023 1,667 2 View

Human-derived cell line comparable to DPSCs?

Hello. I am looking for a cell line with similar properties to human Dental Pulp Stem Cells. DPSCs have the ability to differentiate into odontoblasts in culture. All commercially available cell...

03 June 2021 7,295 0 View

Hayes model 1; model significant, interaction not?

Goodevening. I did a simple moderation analysis (model 1; relation from X on Y with examine the influnce of 1 moderator). If I see the output, my model is significant but my interaction not. What...

15 March 2021 8,036 4 View

What non-parametric alternative can I use for a repeated-measures ANOVA for 2 groups and 2 timepoints?

Hello, I have a question regarding non-parametric alternatives for a RM-ANOVA. In my study I had two groups that were measured on two timepoints (pre-post measurements only). I was planning to...

11 February 2021 8,899 7 View

Can some one help and advise on the Kd calculation from EMSA experiments?

Dear all, I am recently doing EMSA and I have doubts regarding the calculation of Kd. Since I am using ethidium bromide for revelation, I had to use high quantities of DNA (40uM). Moreover, I...

13 May 2019 9,820 5 View

Difficulty growing Listeria monocytogenes (ATCC 19112) biofilms

Hello everyone, I am having difficulty growing Listeria monocytogenes biofilms using the Calgary Biofilm Device (CBD) for the purpose of completing a MBEC assay. It seems like the biofilms are...

26 June 2017 8,196 3 View

Has anyone experienced a problem of DNA degradation after cleaning DNA with the Genomic DNA Clean up and Concentrator kit from Zymo ?

We have been cleaning genomic DNA after a WGA step with the Zymo kit and the samples showed nice clean bands of high molecular weight on an agarose gel immediately after the DNA cleaning. We then...

12 April 2016 398 2 View

How to measure a team member (individual) performance in greening projects. Are there KPI, metrics, or frameworks that exist for easy calculation?

I am trying to find the ways and means that already exist to measure or calculate a team member's (individual) performance in greening projects. Are there any KPI, metrics, or frameworks that...

29 September 2015 397 3 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

02 August 2024 9,518 2 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View