Hi,
In my last experiment, I lysed RBC in a whole lung pellet with ACK. Even when I leave it for longer than 1 minute/ sample (protocol in my lab), the pellet is usually quite white. This time I noticed something grey at the bottom. Then I counted the WBC towards the end for my different groups I noticed there was a lot of debris. I'm not sure if it's the ACK or other enzymes in the lung digestion or the treatment I provided to the mice (I have never seen this until now). Any suggestions/guidance would be appreciated
This type of color is pretty common for human lung samples, and is then likely related to exposure of environmental factors. Did you start treating the mice with a new substance?
Hi Joakim Dahlin , sorry for the delay and thank you for your answer. This is a mouse sample. In previous experiments where I worked with ACK handling mice samples, I hadn't seen that color before. The treatment was my usual in vivo model that I had been using for over a year now. We decided to discard that ACK and get a new one and we haven't experience this ever since :)