Is my initial AP FRET set up suitable?

Hi all! I want to use acceptor photobleaching FRET to look at a potential interaction between two proteins of interest. We have a Leica SP8 in my lab that I have used to try and get an idea if...

09 March 2020 2,349 2 View

Is serum diluted for clinical human Anti-idiotypic ELISAs?

I am working on an anti-idiotypic ELISA for human clinical trials and would like to know if human serum is diluted in these assays or used neat. My ab of interest is an IgG4.

01 August 2018 4,708 4 View

Housekeeping proteins/loading controls for a Western Blot of E. coli cell lysates?

18 July 2017 7,679 5 View

What's your favourite mCherry antibody for immunoprecipitation (IP)?

15 January 2017 5,879 3 View

What is the best way to measure glyphosate?

I am looking for either a lab to do the testing or a relatively simple-to-use kit to do it myself. Can it be done on whole body tissues? What quantity of tissue is required? How expensive is this...

03 February 2016 1,367 3 View

Can anyone offer advice on sarkosyl insoluble tau preparations?

25 January 2016 6,634 1 View

Does anyone know if Santa-Cruz Biotechnologies CRISPR/Cas9 plasmids can be transfected with reagents other than the UltraCruz Transfection Reagent?

03 November 2015 5,360 2 View

Does anyone know if CypHer5 mono ester dye is stable after fixation?

I'm hoping to use CypHer5 mono ester dye to label bacteria, then infect cells with the labeled bacteria. I need to do a fixation step before microscopy, and I was wondering if the dye will be...

18 June 2015 2,581 2 View

Can anyone help me solve EMSA competition, shift and supershift problems?

I am trying to do EMSAs and I am using a consensus and mutant oligo specific for my protein which I ordered from Santa Cruz and then a custom biotin labeled oligo (I have annealed sense and...

14 December 2013 2,352 8 View

First publication of each type of proteomic LC-MS experiment?

I have a somewhat strange discussion to start! A reviewer has asked that we include references for the first time each proteomic mass-spec technique we cover in a review was used. I've been...

01 January 1970 3,224 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

22 January 2021 3,424 3 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Detection of insect eggs in stored and processed products?

Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below...

15 January 2021 6,362 5 View