I am trying to make a G4 oligo with a partial double strand with a biotinylated oligo for smFRET. I started losing G4 FRET in 2 days each time I am making the oligo although I am storing it at 4C with 150mM NaCl or 100mM KCl. And now I am not even seeing anyspots on the slide after attaching the structure, even if I am making fresh oligo. Instead I am seeing some bright spots. Can anyone help?

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