How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

Is my GFAP staining in right position?

I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...

15 February 2021 8,982 1 View

Has anyone measured split YFP in a Tecan (Bimolecular Fluorescence Complementation)?

Hello, I've been trying to measure split YFP-constructs using a Tecan. The problem is, i can detect YFP as a whole construct without any problems (so the excitation/emission wavelengths are fine)...

15 December 2020 2,209 1 View

What is the value of dynamic viscosity and bulk viscosity of extracellular matrix (ECM), nucleus, and cytoplasm of cell?

Hi, I am trying to create a histology based finite element model of human soft tissue. In order to model the viscoelasticity at the cellular level, I need to know the dynamic and bulk viscosity of...

19 October 2020 8,729 1 View

How transfected DNA is transported into the nucleus, to get stable integration?

What kind of mechanisms are involved in the integration and transportation of transfected DNA/Gene to the nucleus?

08 October 2020 2,329 2 View

What is the best way to detect ATF4 activation? And ATF6?

To detect by WB, I would have to isolate the nucleus fraction, right? But I did whole protein isolation. Has anyone detected ATF4 or ATF6 by histology? Thank you

17 September 2020 2,973 1 View

Why COS 7 Cells change its morphology to round shape (rounded nucleus and peri nucleus cytoplasm) after Transfection?

I have transfected COS 7cells to express Protein-GFP conjugate, transfection rate was very low (20-30 %) , but the cell morphology changed, many of the cells appear round, (nucleus round too)...

15 September 2020 5,324 3 View

Why does mitotracker red stains the nucleus?

I'm working with NT2 cells and I use mitrotracker red (400 nM) to stain the mitochondria. I incubate it with cell medium for 30 min, fix with 4% PFA, permeabilize with PBST (0.2%-0.5% Triton...

11 August 2020 8,076 3 View

How should I design the N-terminal VC plasmid of BiFC assay?

I'm going to detect protein-protein interaction with BiFC assays and my target proteins are ER retention with KDEL motif. so I want to fuse VC to the N-terminal of my protein. My question is if...

22 July 2020 3,456 1 View

How can you use the fluorescent intensity of mCherry to calculate BiFC efficiency from split mVenus if the two proteins have different brightness?

I am starting a bimolecular fluorescence complementation (BiFC) assay. In the same vector, there will be 'protein A' (A) fused to 1/2 mVenus in one cassette and in another cassette there will be...

17 July 2020 1,317 1 View