We want to create a semi-random expression library (around 30aa). This could of course be done by using poly-N oligonucleotides, but there is a newer technology where the oligo is synthesized using trimeric nucleotides (codons). This has advantages since you then can choose not to introduce stop-codons, avoid certain amino acids and make customized mixes of codons for certain positions in the oligo.
My question is has anyone experience with these? How long oligo did you order? Any company to recommend?
Thank you.
we have ever constructed 7 libraries using the trimer codon. if yo want to order the trimer codon oligo, please contact me by email: [email protected]. I will provided you the name of the company.
Metkinen Chemistry provide synthesis of the Trimer phosphoramidites as well as Randomized Oligonucleotide Libraries using them. The length of the oligonucleotide up to 100 b, but number of randomizations is limited to 10-14. You can get more information at www.metkinenchemistry.com
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Methods
- Cloning