Xiang-Bin Wang

3 Questions 17 Answers 0 Followers

Questions related from Xiang-Bin Wang

Does methylation affect the CMV promoter in large scale expression of mAb?

We have established several cell lines for expressing mAb with GS/CHO systems. The expression level decreased obviously in > 50L bio-reactors. Some papers say that it's because of the effect of...

09 September 2014 264 5 View

Does anybody know the replication mode of plasmids in yeast?

We want to express more than one protein in yeast. We want to try a one plasmid or two plasmid system. Does anybody know anything about the two plasmid system?

09 September 2014 879 7 View

I want to use CAS9 systems to knock in expression cassette into CHO cells to make stable cell lnes. how do I choose the hot spot for knocking in?

Cas9 is a good genome editing tool. I want to make stable cell lines using CAS9 knocking in tech. I don't know how to choose hot spot for inserting. Does anybody know about it?

09 September 2014 7,264 1 View