This vibratome seems to have a great reputation, however, I am struggling to find if anyone has used it to record from adult Purkinje neurons in acute brain slices (from mouse). Compared to many other cell types, Purkinje neurons are tricky to keep alive and record from. The studies I have looked at (with Purkinje neuron recordings) utilize leica vibratomes. I am considering the leica 1200S and the VF-310-0Z. It's amazing that the VF is less than half the price of the Leica 1200S. If you have any experience in this regard, please let me know!
Thanks!
Joey
Hello Joey,
Have you found any answer? What is your experience, now, a few months later? We will be doing thalamic brain slicing of adult (approximately 1 yr old) mice. We are considering the compresstome VF 310-Z from , as well as the 7000 SMZ from Campden instruments. What do you feel have been the pros and cons of using agar, as is needed with the compresstome? If you are willing to share advice, or discussions, or thoughts regarding selecting a vibratome, please do message me: [email protected]. Or post here.
Thank you kindly,
Sonya Dave, PhD