Nabil Aj

6 Questions 4 Answers 0 Followers

Questions related from Nabil Aj

Has anyone used the TUNEL assay for 3D bio-printed models cell cultures?

Has anyone used the TUNEL assay for apoptosis detection in 3D cell cultures, such as 3D bio-printed models? I'm planning to construct 3D bio-printed models from U87 glioblastoma cells and study...

17 August 2024 4,450 0 View

How to calculate the amount of weight of free-drug for encapsulation efficiency calculation?

I used 15:1 ratio to mix exosomes & Temozolomide drug (300 μL Exos + 20 μL TMZ). My TMZ stock solution is 1 mg/ml, so 20μl = 20μg TMZ. Then, I sonicated/incubated the mixture to load the TMZ...

15 August 2024 5,677 0 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

24 July 2024 4,781 4 View

How to dissolve Temozolomide drug?

I have 500 mg of Temozolomide powder. So, how to dissolve TMZ properly for stock solution (10 mg/ml)? Can I dissolve it in distilled water or PBS instead of DMSO? I will use this drug and load it...

08 July 2024 2,769 2 View

How to load Temozolomide (TMZ) drug into exosomes using incubation? Can I use Tween-20 too for facilitate the drug loading?

Hi, I want to load TMZ drug into extracellular vesicles (Exosomes) using incubation and sonication. I'm still stuck doing the incubation one. For the method, I mixed the TMZ and exosomes in the...

08 July 2024 9,951 0 View

Why my unstained, isotype (IgG) and positive samples (U87 cells) have same histogram results?

Hi, I just want to ask regarding flow cytometry method for indirect staining of U87 cells (Glioblastoma) as I'm still quite new in using this method. My objective is to observe the expression of...

24 March 2024 8,937 1 View