Has anyone used the MirTrap system (TAKARA/Clontech) for identifying microRNA targets?
I m trying to design a two-element microstrip patch antenna array at 2.95 GHz fed by a coaxial probe and a 3-port divider as I show in the attached files. The antennas impedence at the feed point...
12 October 2023 4,146 5 View
Hi. I am conducting and ARDL cointegration analysis. I have a mix of I(0) and I(1) variables. I have seen previous posts that refer to an impulse response analysis complementing ARDL. Since i...
02 November 2022 3,856 1 View
Fuzzy TOPSIS Fuzzy VIKOR
07 February 2022 6,991 2 View
Hello everyone, I am performing reporter gene assays on some uORF mutants vs my WT 5' UTR of my gene of interest. I am doing double transfection on HEK293T cells, using Nanoluc as a test reporter...
22 November 2020 2,964 5 View
Dear Yulia, we are trying to contact Phil and you, but both your e-mails return back our messages...:-( Can you please send us your new email addresses or respond directly to my e-mail:...
02 July 2020 1,989 0 View
I have found 5 included studies for my meta-analysis with binary outcome. Two of them present the results as n/N (n: events; N: non_events patients), while the other 3 three as OR (95% CI). Is...
09 April 2020 9,477 7 View
I want to measure fatigue/sleepiness while driving with a wearable that provides a fatigue/sleepiness score to the drivers in real-time. Therefore, I cannot use devices that collect data that...
15 March 2020 3,727 4 View
Hello, I need to compare two different states of performance for IoT devices. State 1: Under Attack State 2: No any kind of attack. I can easily use honeypots to simulate the incoming traffic...
06 March 2020 7,085 3 View
My project concerns analysis of the microbial community of sediments from aquaculture systems in concrete tanks supplied with fresh sea water. We followed a molecular approach through PCR cloning...
25 July 2018 1,054 2 View
I want to run an experiment with pilots flying a simulated flight. I need to collect data about their performance (accuracy of handling the joystick, speed etc). Do you know if there is a flight...
26 June 2018 2,713 23 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
Some sources say it is treated as Newtonian as ethylene glycol has a higher viscosity than water, which affects the flow characteristics in simulations. But some also say it is non-Newtonian.
09 August 2024 2,111 2 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View