Inconsistent results after plasmid re-purification from frozen stock?

Hello everyone, I am performing reporter gene assays on some uORF mutants vs my WT 5' UTR of my gene of interest. I am doing double transfection on HEK293T cells, using Nanoluc as a test reporter...

22 November 2020 2,746 5 View

Forest plots of meta-analysis with odds ratio?

I have found 5 included studies for my meta-analysis with binary outcome. Two of them present the results as n/N (n: events; N: non_events patients), while the other 3 three as OR (95% CI). Is...

09 April 2020 9,315 7 View

Which wearable (e.g. smartwatch) should I use to get real-time fatigue/sleepiness scores?

I want to measure fatigue/sleepiness while driving with a wearable that provides a fatigue/sleepiness score to the drivers in real-time. Therefore, I cannot use devices that collect data that...

15 March 2020 3,527 4 View

Can I simulate IoT device activity using Honeypots or any other Tool?

Hello, I need to compare two different states of performance for IoT devices. State 1: Under Attack State 2: No any kind of attack. I can easily use honeypots to simulate the incoming traffic...

06 March 2020 6,947 3 View

DGGE bands enhancement?

My project concerns analysis of the microbial community of sediments from aquaculture systems in concrete tanks supplied with fresh sea water. We followed a molecular approach through PCR cloning...

25 July 2018 943 2 View

Anyone know how to do GC peak separation?

10 December 2014 7,644 26 View

How has technology impacted the factors of the instructional process, e.g. in the 'Berlin Model' & its phases, e.g. in the 'ADDIE Model'?

23 August 2014 1,029 4 View

What is your experience/opinion/reference regarding code-switching in education?

01 August 2014 4,809 59 View

Rehydration buffer for 2D samples.

I am using the following rehydration buffer for my 2D samples. Do you think any of the components may be altered/ become unstable after long period of storage at -20oC and thus affect the...

05 December 2012 9,738 6 View

Is it possible to freeze an antibody mix?

Is it possible to freeze a primary and secondary (Jackson ImmunoResearch) antibody mix several times and reuse it? Will the antibodies still retain their same affinity after a long period of time?...

15 October 2012 4,955 17 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Statistics: include groups of respondents made by one person for analysis?

Good morning, I could not find literature about this so I am asking. I am organizing data for a questionnaire (N=100) that foresees some personal questions like level of education. For the aim...

25 February 2021 2,090 4 View