Inconsistent results after plasmid re-purification from frozen stock?

Hello everyone, I am performing reporter gene assays on some uORF mutants vs my WT 5' UTR of my gene of interest. I am doing double transfection on HEK293T cells, using Nanoluc as a test reporter...

22 November 2020 2,746 5 View

Forest plots of meta-analysis with odds ratio?

I have found 5 included studies for my meta-analysis with binary outcome. Two of them present the results as n/N (n: events; N: non_events patients), while the other 3 three as OR (95% CI). Is...

09 April 2020 9,315 7 View

Which wearable (e.g. smartwatch) should I use to get real-time fatigue/sleepiness scores?

I want to measure fatigue/sleepiness while driving with a wearable that provides a fatigue/sleepiness score to the drivers in real-time. Therefore, I cannot use devices that collect data that...

15 March 2020 3,527 4 View

Can I simulate IoT device activity using Honeypots or any other Tool?

Hello, I need to compare two different states of performance for IoT devices. State 1: Under Attack State 2: No any kind of attack. I can easily use honeypots to simulate the incoming traffic...

06 March 2020 6,947 3 View

DGGE bands enhancement?

My project concerns analysis of the microbial community of sediments from aquaculture systems in concrete tanks supplied with fresh sea water. We followed a molecular approach through PCR cloning...

25 July 2018 943 2 View

Anyone know how to do GC peak separation?

10 December 2014 7,644 26 View

How has technology impacted the factors of the instructional process, e.g. in the 'Berlin Model' & its phases, e.g. in the 'ADDIE Model'?

23 August 2014 1,029 4 View

What is your experience/opinion/reference regarding code-switching in education?

01 August 2014 4,809 59 View

Rehydration buffer for 2D samples.

I am using the following rehydration buffer for my 2D samples. Do you think any of the components may be altered/ become unstable after long period of storage at -20oC and thus affect the...

05 December 2012 9,738 6 View

Is it possible to freeze an antibody mix?

Is it possible to freeze a primary and secondary (Jackson ImmunoResearch) antibody mix several times and reuse it? Will the antibodies still retain their same affinity after a long period of time?...

15 October 2012 4,955 17 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Can someone help me with this error that I am getting while estimating a VAR model in R?

I am using annual time series data to estimate a VAR model in R. However, when I run the command to find the optimal lag length, I get the following error: Fehler in VARselect(data, lag.max = 10,...

18 February 2021 9,647 3 View

Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

10 February 2021 6,090 3 View

How to check normal distribution in a cell viability test?

To choose whether a parametric or a non-parametric test should be used to compare data on viability that emerge from an assay such as MTT, one should establish if the data are normally...

02 February 2021 1,733 4 View

Reliability of the model created by RSM (Response Surface Methodology)?

When you create a model with RSM, even if the R2 value is large and the result of the analysis of variance is significant, if you look at the individual explanatory variables, if there are many...

22 January 2021 454 1 View

When sequencing a plasmid to confirm transformation has been successful, would I have to sequence the full plasmid?

I have transformed some DH5alpha cells with point mutated proteins placed in pcDNA plasmid and extracted the plasmid; now I have to send the plasmid for sequencing for confirming the presence of...

19 January 2021 5,842 3 View

Can I use breaking series as a regressor in a VAR model?

Context: I have two series, price and sales. Sales is mean-reverting stationery but price is stationary only after controlling for an intercept break. I want to set up a 2-equation VAR model...

14 January 2021 6,824 12 View

Model reduction using Machine Learning techniques (...see description) ?

If we have a SISO dynamical system, for reduced order approximation using ML algs, do we need to take (frequency/ time domain) samples of Impulse response/TF Then to approximate this input/output...

08 January 2021 7,720 3 View

Response Surface Methodology?

I'm using 3 different light intensities (constant values 15,30,45 W) for the wastewater treatment of an organic compound and optimizing the parameters (pH, catalyst dose and light intensity)...

31 December 2020 2,377 4 View

Do you think the current COVID-19 vaccine would be effective against the new variant?

Do you think the current COVID-19 vaccine would be effective against the new variant?

22 December 2020 5,025 9 View