09 September 2014 1 5K Report

Has anyone used the Miltenyi Biotec GFP/HRP antibody (130-091-833) for ELISA? I am attempting to use it as a detection antibody for ELISA and it is not working with my GFP construct. I have tested using different antibodies to confirm if my protein of interest is present but I need the GFP/HRP antibody to detect it. Anyone know how to troubleshoot this antibody?

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Just a generic question, what is the average half-life of a typical membrane protein? If you have a review off hand that could be sent to me, that would be greatly appreciated.

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Where can I find used or demo microscopes?

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Can food grade CO2 be used in place of medical grade CO2 for a cell culture incubator? Our lab is running out of medical grade CO2 and all we have on hand is food grade. We can't order more...

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What is a good CDC42 Inhibitor?

Hi, I need a good inhibitor for CDC42. I am new to working with this protein and wonder if anyone knows what inhibits it well. Thank you! Chuck

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Does anyone have a dominant negative Wiskott Aldrich Syndrome Protein Mutant they would be willing to share?

I am looking for a dominant negative Wiskott Aldrich Syndrome Protein Mutant. I would prefer if it has an mCherry tag. Our lab would pay for shipping and acknowledge your contributions to any...

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Primer size for deletion mutagenesis using overlap extension PCR?

I am trying to delete 30nt regions of a plasmid but have been unsuccessful in my PCR using overlap extension PCR. My primers range in size between 23 and 36 bp in length. I fear that my deletion...

11 December 2017 4,766 6 View

What is the minimum amount of DNA required to be loaded on a gel for a gel extraction/purification?

I am trying to purify DNA from a gel but yields are seemingly too low. I have loaded ~500ng of DNA onto my gel, run it, and cut it out and purified it using a Quigen Gel Extraction kit. I am only...

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How do I cut out a section of a plasmid?

Hello, I am relatively inexperienced with certain molecular biology techniques. I have a gene that has been cloned into a plasmid for me. I would like to leave the N and C Terminal domains intact...

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Can someone help me with mutagenesis troubleshooting?

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