We need to use this genotoxic test in Chlamydomonas reinhardtii, for that we want to know which is the best method to eliminate the cellular wall of the cells and more information about the analysis using flow cytometry.
Micronucleus tests (MNT) are best used in animal cells and animal based MNT are widespread world over. Using MNT in plant cells to understand the geno-toxicology is poorly known.
I have used MNT in fishes to assess geno-toxic effects of various chemicals.
Micronucleus (MN) assay is very easy and rapid test for the assessment for genotoxicity of any compound in any models (Cell lines, blood, animals, etc.,). The MN assay needs to be standardized against any models. For flow cytometry analysis, use the following references to under stand the basics.
1. Nusse M and Kramer J. Flow cytometric analysis of micronuclei found in cells after irradiation. Cytometry. 1984; 5: 20-5.
2. Schreiber GA, Beisker W, Braselmann H, Bauchinger M, Bogl KW and Nusse M. An automated flow cytometric micronucleus assay for human lymphocytes. Int. J. Radiat. Biol. 1992; 62: 695-709.
3. Laingam S, Froscio SM, Humpage AR. Flow-cytometric analysis of in vitro micronucleus formation: comparative studies with WIL2-NS human lymphoblastoid and L5178Y mouse lymphoma cell lines. Mutat Res. 2008; 656: 19-26.
Although Micronucleus tests (MN) are best used in animal, you can serve the manuscript:
Evaluation of the migration of mutagens/carcinogens from PET bottles into mineral water by Tradescantia/micronuclei test, Comet assay on leukocytes and GC/MS
DOI: 10.1016/S0048-9697(02)00349-2
http://www.sciencedirect.com/science/article/pii/S0048969702003492#
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