Has anyone used irradiated pig PBMC to stimulate human PBMC?

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How to display grain boundaries in RVE model?

Many literatures have shown that the RVE model shows the grain boundaries between different grains. How can this be achieved using DAMASK+paraview?

21 July 2024 9,224 1 View

Gaussia Luciferase for Bioluminescence Tumor Monitoring in Comparison with Firefly Luciferase. Which is better?

Been doing searches to find the best paper to start understanding the advantages and disadvantages of each technology: Gaussia Luciferase for Bioluminescence Tumor Monitoring in Comparison with...

29 June 2024 9,856 1 View

Is it better to use 2 intact classes for the quasi-experimental study?

Hi , in my study I will compare the effectiveness of Al generated feedback and traditional feedback in writing lesson.It will be a quasi-experimental design,but l have 3 classes not two.What...

25 June 2024 6,244 3 View

How to distinguish the origin of sulfides in subduction zone interface rocks?

If we find sulfides in subduction zone interface rocks, how can we distinguish whether these sulfides are related to crust-derived fluids or originate from the original mantle wedge? Especially...

15 June 2024 654 1 View

Help with mounting meningeal tissues with Prolong Diamond?

Hello! I've recently been trying to optimise immunofluorescent staining of mouse meningeal tissue, and I'm having trouble mounting it just because it's so thick. We use prolong to mount and...

05 June 2024 624 2 View

How to determine sample size using sequential analysis for a registered report in psychology?

Hi Research Gate people! I am trying to determine the sample size, power and alpha boundary needed for my interim analysis for a registered report in a psych journal. I have read the paper by...

28 April 2024 3,444 2 View

Categorical X in mediation formulation and analysis?

X=categorical IV (X1, X2) M1,M2,Y=continuous variables First: 1. compare X1 and X2, and hypothesize that X1 will predict higher Y 2. X1 will predict M1 3. M1 will predict M2 4. X1 will predict...

26 April 2024 6,207 0 View

How do I compare and plot Poisson's ratio against time under different loading case using strain values?

I've previously conducted a shearing test against a limestone rock sample using a drilling machine in experiment 1. To increase the roughness and asperity contact, quartz particles were attached...

20 April 2024 3,104 0 View

Solution to Enhance RNA Extraction Efficiency from Zebrafish Larval Brain and Gut Tissues?

I am Nicholas from the University of Malaya. I have been performing RNA extraction from whole zebrafish larvae for some time using NucleoZOL as my lysis buffer and passing my samples through...

16 April 2024 3,576 0 View

Why do I keep getting high Safety Factor on Plaxis 2D?

I keep getting high safety factor and tried to change multiple properties.

25 March 2024 594 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View