I would like to use CypHer5 coupled with actin anti-body to study endocytosis of apoptotic sperm in mosquitoes. Does anyone have experience with CypHer 5 dye?
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...
08 July 2024 3,150 2 View
Do you know systematic reviews and/or meta-analyses that have chosen to exclude articles from (or suspected to be) predatory journals/publishers in their work ?
24 March 2024 4,941 3 View
Is it possible to keep them in -80 freezer or should we keep them in liquid nitrogen?
17 July 2023 2,846 1 View
We're looking for a complete system for producing anodized aluminum oxide (AAO) substrates from self-supported aluminum foil?
12 June 2023 3,526 2 View
Dear members, As you know, the identification of urinary tract infection by culture is crucial for the diagnosis and treatment. However, traditional methods of culture identification can be...
04 May 2023 7,178 3 View
Hi, I need humanized mouse models for flavivirus infection but I do not have the skills to produce them. Do you know of a company that can do it. Thank you very much
20 February 2023 3,058 0 View
Hello everyone, I'm currently doing some DTA analysis on two phases of the same ceramic material, one is fully crystalline and the other fully amorphous. When I work with the crystalline one I...
03 July 2022 8,061 3 View
I would like to perform some microscopy experiments on yeast zygots. Unfortunetly zygots are clumping together which is not ideal condition for my experiment. I tried to add 0.1% of Tween80 with...
01 March 2022 8,606 2 View
Hi, I'm a fish biologist and I'm interested in assessing the uncertainty around the L50, which is the (sex-specific) length (L) at which you expect 1 fish out of 2 (50%) to exhibit developed...
21 November 2021 173 11 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View