I did a blot with HMG Co-A Reductase primary antibody (Cat # BS-5068R) purchased from Bioss (Woburn, Massachusetts, USA) for mouse liver tissues.

I am getting a band at 70kDa, though the protein is actually weighing 97kDa. Further there were 3 nonspecific binding sites at 35, 40 and 45kDa on my membrane. Since Bioss doesn’t show any image of a western blot on their data sheet it’s bit confusing.

Out of the 4 proteins I blotted for, only on HMG Co-A Reductase membrane showed this kind of results. Therefore I do not suspect the reagents used and the protocol followed which is been used for years in our lab.

Sharing any experience regarding this issue would be highly appreciated.

Thanks,

Susara