Has anyone tried out sekisui diagnostics triglyceride assay kit ?

Susara Madduma Hewage @Susara-Madduma-Hewage

11 October 2017 0 4K Report

Has anyone tried out Sekisui diagnostics triglyceride assay kit to determine mouse liver triglycerides ?

Though the Sekisui protocol is made for automated systems I'm trying to use it manually.

Any suggestions ?

Thanks

Badges
Science topic

More Susara Madduma Hewage's questions See All

Where does phenomenological research fit within the layers or stages of the research onion proposed by Saunders et al. (2018)?

I've selected an interpretivist philosophy along with a qualitative inductive approach for my research. I plan to employ semi-structured interviews as my primary data collection method. However, I...

12 May 2024 7,816 4 View

How can I generate angle restraints using gromacs?

The system that I am working on contains two ligands with a protein and I want to use angle restraints between water molecule and amino acid residue along with the distant restraints.Is there any...

25 September 2020 1,132 0 View

Have you ever got high backgrounds in western blots done with heart tissues ?

Hi, I am doing WB with heart tissues (Rat) these days and having a problem of high background. We have no issue of getting high background for WB done with cells or other tissues, the protocol is...

11 June 2019 2,871 3 View

What are the essential cofactors to perform in vitro assay using alpha/beta hydrolase enzyme ?

Need to perform in vitro reaction with substrates and purified protein (Methyl esterase/alpha/beta hydrolase super family) 1. What are the essential cofactors? 2. Which buffer solution/pH should I...

14 April 2019 9,411 1 View

Measure beta-oxidation of fatty acids?

Hello all, I am looking for a kit or a method to measure beta oxidation of fatty acids (without radio labeling) in tissues (Mouse) and cells (Human). Any suggestions ? Thanks

16 January 2018 8,997 11 View

Getting low fluorescence values for Control group compared to H2O2 group in intracellular ROS detection with DCF-DA probe?

I was trying to detect H2O2 induced intracellular Reactive Oxygen Species (iROS) generation with DCF-DA fluorescent dye (2′,7′-Dichlorofluorescin diacetate, Sigma Aldrich, St. Louis, MO) in HK-2...

09 November 2017 1,675 5 View

Should you call your sample items, participants or respondents in mixed methodological approach?

Intend to use qualitative research method and quantitative research method together ( semi -structured interviews and questionnaires ) for answering two separate research questions . In data...

16 June 2016 2,313 7 View

How do I transfer biogass from a digester to a pressurized cannister?

I am building a small scale digester to test whether it is possible to store the methane produced in a pressurised cannister. I need to find a method that will allow me to fill the pressurised...

15 June 2015 3,714 4 View

Any suggestions on how to measure water flow in saturated wetland soils?

I am looking into a possible experiment to quantify N and P uptake by wetland monocots

07 April 2014 1,672 19 View

Is there a stain other than Triphenyltetrazolium Chloride that could be used on roots with a thick epidermis?

I am looking into the root senescence of wetland plants and need to quantify viability at the beginning on the growing season.

21 April 2013 7,100 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

Can anyone suggest me the questionnaire along with scoring key to assess the mental health wellbeing of the college faculty?

I am Ruthra, and I have been working as a psychologist for the past 10 years. Now, I'm interested in conducting research and I would like to conduct a survey on mental health and wellbeing among...

15 July 2024 4,119 4 View

What are the best ways to automate my job and live without working?

I want to know the best methods to automate my job and live without working?

12 July 2024 5,993 2 View

How can get paper ?

I need a copy of the research titled “An Efficient Automated Multi-Modal Cyberbullying Detection Using Decision Fusion Classifier on Social Media Platforms" An efficient automated multi-modal...

08 July 2024 7,381 3 View

What is the current status of augmented learning in robotic surgery?

I would like to perform a literature review at this time on augmented learning and learning augmented algorithms to enhance performance-guided surgery

06 July 2024 246 1 View

How to fill large gaps in GIS?

Dear colleague, I need help with a GIS issue. The polygons have been separated by roads, leaving space between them (as shown in the attached figure). I'm looking for an automated solution in...

04 July 2024 6,752 3 View