Have you ever got high backgrounds in western blots done with heart tissues ?

Hi, I am doing WB with heart tissues (Rat) these days and having a problem of high background. We have no issue of getting high background for WB done with cells or other tissues, the protocol is...

11 June 2019 2,726 3 View

What are the essential cofactors to perform in vitro assay using alpha/beta hydrolase enzyme ?

Need to perform in vitro reaction with substrates and purified protein (Methyl esterase/alpha/beta hydrolase super family) 1. What are the essential cofactors? 2. Which buffer solution/pH should I...

14 April 2019 9,298 1 View

Should you call your sample items, participants or respondents in mixed methodological approach?

16 June 2016 2,206 7 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

Nuances in the digital twin concept. Opinions?

Hello everyone, I am currently working on the state of the art of the digital twin concept, and after seeing the categorization made by Kritzinger, W. et al. (2018) [Digital Twin in...

14 February 2021 2,107 3 View

What Research is yet left in the field of Automation and Unemployment?

I have been through a number of journals and Research Papers. I wanted to get an idea as to what new areas are still left to be researched in "Will Automation lead to unemployment or it will lead...

09 February 2021 9,332 4 View