I want to separate inner and outer membrane of intestinal and salivary gland nuclei and verify the effectiveness of nuclear membrane separation, although not much information is available. Can anyone give me some advice?
No I haven't tried yet. First doing some literature review to check which one would be the best marker but thank you for your suggestion.
Aneta I believe a hard task to separate the nuclear membranes because they form a cistern, you could isolate the nuclear cisterns, but to separate is really complecated because the vesicles that you obtain always will have both faces. I am sending you a review where you can find specifics and differents types of markers. Good Luck
Thank you very much for your suggestions and article. It is indeed a hard task I am working on. Some papers show the separation protocol but for a different tissue which doesn't seem to work out for my tissue, so I am still looking for a solution.
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