I use PRB1-3 antibody from Santa Cruz and I am afraid it might not work well. The reason why I think this is that I get the same band pattern after the incubation with primary and secondary antibodies as well as secondary antibody alone. I have already changed many parameters: concentration of primary antibody, secondary antibody, concentration of loaded sample, also I've used different tissues, yet none of them seems to be working well. The problem is also that the company doesn't have a positive control for sale so I can not check if the antibody works. Does anyone have any suggestions how to check the antibody? Or why I cannot obtain a band of my protein?

Thanks in advance.