Aneta Bragiel

3 Questions 6 Answers 0 Followers

Questions related from Aneta Bragiel

Has anyone used a method for purification of inner and outer nuclear membranes and good outer-nuclear-membrane marker (app:rat tissue Western blot)?

I want to separate inner and outer membrane of intestinal and salivary gland nuclei and verify the effectiveness of nuclear membrane separation, although not much information is available. Can...

05 May 2014 8,943 4 View

Can anyone help with a signal on the blot without primary antibody?

I have been using ECL mix to detect the optimum primary antibody concentrations for a western blot and found out that even without the primary antibody I get bands though they are quiet light. The...

04 April 2014 2,662 3 View

Has anyone used PRB1-3 antibody from Santa Cruz (sc-169028)?

I use PRB1-3 antibody from Santa Cruz and I am afraid it might not work well. The reason why I think this is that I get the same band pattern after the incubation with primary and secondary...

03 March 2014 9,516 2 View