I want to aspirate plasma, PBMCs, and lysed RBCs out of blood samples contained in microhematocrit capillary tubes. I need a more precise way than just blowing it out. Hamilton syringes might work?
I don't see any problem with plasma or lysed RBC aspiration, although the aspiration of packed RBC will be problematic.
P.S. You must to aspirate very slowly.
That's what we use for plasma and works great. I agree about the packed RBC, it can just be blown out, Hamilton may not be much of a help.
Thanks Zoltan - what gauge? I think 26 g works fine (with a regular hypodermic needle) but just curious what model you have.
50 & 100 microliter syringes with a fixed needle tip. I'll look up the exact model and will get back to you.
As the previous replies note, "it's the only way to go" as it allows virtually no waste of plasma. And, when one's dealing with little birds/animals with limits on how much blood can safely be collected this is invariably important.
This is the model we use:
http://www.hamiltoncompany.com/products/syringes-and-needles/general-syringes/microliter-syringes/700-series/100-microL-Model-710-N-SYR-Cemented-NDL-22s-ga-2-in-point-style-2
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