Blanca Fabiola Fajardo-Fregoso

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Questions related from Blanca Fabiola Fajardo-Fregoso

Has anyone used a Fluorimeter (Jenway) to assess NaFl??

I'm currently using a Jenway Fluorimeter to assess the variations in BBB permeability. But there are certain things that i can't configurate right, (and the manual has not been very helpful). For...

02 October 2017 759 0 View

WESTERN BLOT How do you transfer high and low MW proteins at the same time? I'm trying to get Claudin-5 (~22kDa) and ZO-1 (~220kDa)

I run the electrophoresis in a pre-cast 4-20% gel at 200 V for ~45 min, then i've tried to transfer them to nitrocellulose membrane in the following conditions (always in a cold, wet environment):...

01 January 1970 7,257 6 View

Blanca Fabiola Fajardo-Fregoso

Has anyone used a Fluorimeter (Jenway) to assess NaFl??

*WESTERN BLOT* How do you transfer high and low MW proteins at the same time? I'm trying to get Claudin-5 (~22kDa) and ZO-1 (~220kDa)

WESTERN BLOT How do you transfer high and low MW proteins at the same time? I'm trying to get Claudin-5 (~22kDa) and ZO-1 (~220kDa)