According to ATCC, it is a clone from 3T3-L1 "to ensure close to 100% differentiation to adipocytes and great response to insulin". But I can't find many articles using this cell line. Does anyone have any experience of using this cell line for adipocyte differentiation? Typical 3T3-L1 cells cannot differentiate well after P10. Would this 3T3-L1 MBX retain its differentiation potential for longer?
Thanks everyone!
Cells were maintained in a humidified chamber with a 5% CO2 and 95% air mixture at 37°C in DMEM containing 10% fetal bovine serum (Invitrogen, USA). Differentiation to adipocytes was induced by culturing the cells in differentiation medium [DMEM, 10% FBS, 2 mM insulin, 1 mM dexamethasone, 0.25 mM 3-isobutyl-1-methylxanthine (IBMX)] for 2 days. The medium was replaced with DMEM supplemented with 10% FBS and 5 µg/ml insulin for additional 2 days. Thereafter, the medium was changed every day with DMEM supplemented with 10% FBS.
It's hard to find out much about these cells, so I would be cautious. Clearly it has something to do with the MBX-102 TZD-like compound, so perhaps these cells have a boosted PPARy expression/activity? Regular 3T3L1s do what you need them to do, and although it's a pain to keep restarting young cultures all the time, I would stick with them until more is known about these cells.
I am looking to use these cells too.... Any updates on how these cells behave as compared to the normal 3T3L1 cells?
We are currently using both cell lines and found high rate of differentiation for 3T3MBX (more than 90%). Thus they are more homogeneous than 3T3L1. In culture their proliferation rate is lower than 3T3L1 cells and they can be differentiated into fully (i.e. unilocular adipocytes) . We describe an application for lipid sensing of intestinal barrier oleic uptake in:
Adipocytes as lipid sensors of oleic acid transport through a functional Caco-2/HT29-MTX intestinal barrier.
Berger E, Géloën A.
Adipocyte. 2019 Dec;8(1):83-97. doi: 10.1080/21623945.2019.1580842. Epub 2019 Mar 23.
PMID: 30905315
We've started trying 3T3-L1 MBXs, they do seem to accumulate lipid slightly better than 3T3-L1s. However, in 2D culture (after confluency and initiation of differentiation) they seem to be becoming aggregated/seem to be compacting their monolayer. Emmanuelle Berger did you experience this when working with the MBXs?
We've also noticed a more spindley morphology compared to the 3T3-L1s.
[Pics are 3 days differentiation in DMEM + 10% FBS + insulin + IBMX + dexamethasone + rosiglitazone, followed by 2 days in maintenance media (DMEM + 10% FBS + insulin + intralipid)]
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