Can anyone suggest me a solvent ratio to increase the gap between these two spots?

After column chromatography I've got these two spots together. And tried a number of solvents ratio to increase the gap between these two spots for further column chromatography but failed.

10 October 2020 1,367 4 View

What is the appropriate length of packed silica for column chromatography?

In the case of column chromatography of plant extract, which is the appropriate length of packed silica of a one-inch diameter and 18 inches long column, and what speed should be used for solvent...

29 September 2020 4,818 8 View

Should I use glass vials for mouse organ preservation for short term or plastic vials are good enough for short term storage?

I have noticed many researchers use both glass and plastic vials for mouse organ preservation. Which one is better in case of short term storage?

19 July 2020 4,928 4 View

Does anyone know which software is available to download that can visualise .svs histology slides for a mac computer?

View histology svs files on a mac, free to download software.

14 July 2020 5,719 1 View

I am a colege student in a institute teknology, now i begin to make a research. And i confuse, iam not have idea. Please some one help me..?

Please give Me idea to begin my reserch in electricitian. Thankyou very much

03 January 2020 8,855 2 View

How to measure the distance of chromaticity coordinates and efficiency?

15 June 2017 8,321 3 View

Blackhole MANET OPNET

How to implement a malicious node over OLSR and GRP in MANET to perform black hole attack using OPNET simulator?

30 May 2017 5,758 3 View

Can anyone direct me to the references for the 2-item factor (latent construct)?

I'm looking for references/ published work supporting as well as rejecting a 2-item factor (latent construct). It's well known that 3 item per factor is considered reliable, at minimum.

19 October 2014 2,668 13 View

Isolation of low polar compound from hexane fraction of plant extract

What type of solvent ration is appropriate for the isolation of low polar compounds from hexane fraction of plant extract by traditional silica(60-120 mesh) packed column chromatography?

01 January 1970 2,525 8 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View