Has anybody a protocol to determine the AChE activity in larvae zebrafish brain?

Even following the instruction of Karnovsky and Roots (1964), I still do not know how long I should fix the larvae and in what fixative. Could anyone help me? Thank you!

14 January 2021 385 2 View

Women learning styles/ Women cognition development- Any suggestions for research based on Belenky's work?

I'm looking to expand my sources on the topic

10 December 2020 5,426 17 View

How can I get an homogeneus suspension of Bacillus subtilis?

I have different plates with different strains. The next step consists of doing a suspension of each microorganism. I do not have any problem with E. coli, S. aureus, etc, but I do with B....

29 January 2020 8,348 1 View

Flow cytometry -advance pipeline & compensation?

Hii! So I designed for my next experiments a quite complex panel and I have a few questions about how better proceed for treating my Abs and building my compensation. So I have a Fixable...

14 November 2019 6,590 3 View

How to avoid "U" shape RNA bands in LE agarose gels?

15 April 2019 9,668 2 View

Problems with biological indicator in autoclave, why?

I have problems with the new autoclave. We checked the sterilization process and realized that the biological indicator (B. stearothermophilus) grew after the sterilization. The technician put...

23 January 2019 9,796 3 View

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I obtain few astrocytes after the subculture step. Some astrocytes remain adhered to the culture dish surface after washing with PBS (Without Mg and Ca), incubating 15 min with PBS (Without Mg...

12 November 2018 4,172 2 View

Number of passages for the mouse choroid plexus cell line ECPC?

08 April 2018 8,872 1 View

How to connect routers to an existing network?

Hi! I have a network created with WiFi modules and I want to connect routers to that network so they can forward the messages to the corresponding module. If someone could give me some information...

31 August 2016 5,813 6 View

How to start implementing a VANET prototype?

Hello! I would like to implement a VANET prototype in an Arduino or something similiar and I don't know how to start. If someone could give me some advice on what do I need to start implementing...

13 June 2016 9,574 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View