31 January 2017 0 7K Report

I'm wondering if its possible to measure acetylcholine from drosophila brains using HPLC-EC. How many brains would I need? Should I microwave the brains first to get rid of acetylcholinesterase? Should I homogenize in PCA as you would a mouse/rat brain? Etc.

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how do i set one up? What do I include?

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Why and how to solve unmatched forward and reverse sequencing result by Sanger sequencing?

I’m currently working on polyglutamine disease by Sanger sequencing to count the CAG repeats. However, since my targeted genes involve CAT or CAA interruption in the CAG repeats, their...

01 July 2024 8,328 5 View

Attempting an antibody titration?

Is it ok to titrate new antibodies at a low cell concentration like 100,000 cells per 100 uL instead of 1x10^6 cells per 100 uL? I want to titrate four new antibodies at 4-5 different dilutions to...

02 May 2024 7,613 1 View

How can I correct an error in ResearchGate?

They say we were cited by an article but we weren't. "Karen, we found a recent article that cited you:Relationship of serum total cholesterol and triglyceride with risk of mortality in...

15 April 2024 3,993 0 View

How much PCR product to load on agarose gel?

I have the PCR products of two bacterial genes (gyrA=1024 bp and rpoB=808 bp) and I want to know how many microliters I should add in the agarose gel?.

27 March 2024 2,798 7 View

Thermo Xcalibur error who can help to solve?

All working GC Thermo 1310 simple configuration GC FID in first line. Last week error apear when starting the program that Thermo aqusition service not runing. Reinstalation of Win 10 operation...

06 March 2024 6,701 8 View

I measured Cronbach's alpha on the same scale 4 separate times; 3 were in the .80s and one was .46. Why would this be?

I measured Cronbach's alpha on the same scale 4 separate times; 3 were in the .80s and one was .46. Why would this be?

30 December 2023 7,677 0 View

What are the effective practices and challenges of teachers in obtaining the TPACK?

Teachers are expected to gain knowledge in pedagogical, content and technological aspects to be effective in the field of teaching. Yet, there are teachers who have a hard struggle to become fully...

06 October 2023 7,580 2 View

Is it possible to mix bacteriological agar with PDA culture medium of any brand?

Hello everyone, I am doing my undergraduate thesis and I need to make medium strength PDA culture medium. For this, I must mix 19.5 g/l of PDA culture medium plus 7.5 g/l of Bacteriological agar....

20 September 2023 7,923 0 View

General Linear Model or Mixed Model?

Hi, My current research question is looking at whether children at 5-8 months of age have memory. To do this, we have run an eye-tracking study doing 3 encoding slides and 1 test slide. in the...

13 September 2023 3,335 1 View

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