Is this method of modeling plastic behavior in Abaqus correct?

I've to model the plastic behavior (Yield Stress vs Plastic Strain) of a polyimide material (Elas_Mod=2500MPa) in Abaqus. The engineering stress vs strain plot is shown as the 3 red curves. I've...

03 February 2021 7,728 3 View

What are the insilico tools available to recognize transcription binding sites on a promoter ?

I have to control the expression of a gene ( in vitro ). For designing an optimal promoter assay, I would like to know more about Transcription factor binding region, enhancer and repressor...

11 January 2021 7,992 3 View

How we can measure the infiltration in soil having gravels more than 30 % ? Please suggest correct way for measurement of infiltration rate ?

How we can measure the infiltration in soil which having gravels more than 30 % . We want to calculate infiltration rate in our research work. Kindly suggest correct way for calculation of...

27 July 2020 829 3 View

Can anyone share experimental manual of Arabidopsis thaliana book?

I am working on this model system. So I am looking a book on this model system which can help me designing experiments and perform them in my laboratory. I will be thankful for you. thanks

08 June 2020 3,157 2 View

How to perform simultaneously structural (Rietveld like) and microstructural analysis using WPPM method implemented in PM2K software for powder XRD?

I am learning Whole Powder Pattern Modelling (WPPM using PM2K) of powder XRD data to estimating microstructural properties. In this approach peak intensities are treated as free fitting parameters...

30 April 2020 7,802 1 View

How does the preprints are different from published article?

What is the way of publishing preprint and where to publish the biological data in a preprint form? Next how to convert the preprints into a research articles?

09 April 2020 139 3 View

Indicator variable - Pooled regression results vs data partition results?

This is the Base regression: Y = b0*X1+b1*X2+error term I am using an indicator variable Z which takes value 1 for years 4 to 6 and value 0 for years 1 to 3. I am trying to analyse the impact of...

05 March 2020 9,865 3 View

What is importance of dew in crop production and productivity in semi arid region of India ?

I am interested to know importance of dew in crop production and productivity in semi arid of India. How it play important role during tress period.

23 February 2020 974 3 View

What is the alternative method to centrifugation to remove acid residues from sediments during carbonate removal process?

I am removing carbonates from clay and sand samples. So, I treat the sediments with H2O2. I need to wash the sediments to remove acid residues. I am thinking of heating the sediments with...

06 December 2019 5,057 6 View

Is any one know about yeast strain which use for stress tolerance experiment like INVSc1?

I work on some stress tolerance genes from plants. I clone those genes in pYES2 yeast expression vector and transform in to INVSc1 yeast strain to perform stress tolerance assay using different...

11 November 2019 2,248 1 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View