How do we formalize peer learning?

22 September 2020 8,688 16 View

Which is the best methodology of research in literary work analysis and comparative literature studies? studies?

I'm developing a project on migration and literature, migration in literary works.... I was thinking about discourse analysis...May anyone suggest some methodologies used in these fields of...

13 August 2020 9,742 25 View

How do teachers develop skills and knowledge in teaching mathematics for student learning?

In view of the above, several questions arise that draw attention to the research on how we should prepare our teachers throughout their profession to teach students the national curriculum, with...

25 July 2020 5,687 23 View

If I sequence the same sample twice, can i add the sequencing depths?

If i sequence the same sample twice (two different illumina runs): sequencing depth of 10 sequencing depth of 20 can I claim a sequencing depth of 30? Seems counterintuitive to me but I haven't...

22 March 2020 907 5 View

What sem approach should i use if i have one measurement equation or latent variable and one structural equation , and also i have longitudinal data ?

Hello, i want to use structural equations modelling given that i'm interested in the effect of a latent variable "disclosure" on firm value, i have questions about how to model this relationship...

14 May 2019 2,442 3 View

What cell types can I identify with my flow cytometry panel?

20 May 2018 9,335 3 View

Microscale Thermophoresis with erythrocytes?

Is it possible to do microscale thermophoresis assays with erythrocytes? Has anyone done it? Thanks!

07 January 2018 2,443 2 View

How can I extract Glut 4 from Skeletal Muscle Lysate?

30 November 2017 4,995 1 View

Does anyone have primer sequences for qPCR for rabbit ALP (bone)?

I have been working with rabbit bone marrow and periosteal MSCs and inducing the cells into the osteogenic lineage using specific differentiation media. When performing PCR (so to eventually do...

26 July 2017 636 4 View

Would you take the time to peer-review my questionnaire on social collaboration?

I just want to spot any inconsistencies, before going into the field. Since the survey is dedicated to a very special target group with specific knowledge, please select always "yes" and then...

01 February 2017 9,203 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View