What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

Which drug is better to modify blood pressure in awake monkey, if I provide it in the food or drink reward?

I need to modify the blood pressure in a monkey while is awake in the recording chamber, and I am planing on provide a drug for that in the food or water used for reward... any advise? Thank you

08 February 2021 9,900 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

Using a nucleosil c18 column, i get no negative peaks in real time and two negative peaks in post run.Software problem?

Simadzu, lc solutions

02 February 2021 1,969 2 View

Any ideas on fast and easy way to stain mucus on intact cell layer?

Dear RG community, We are looking for a fast and easy way to stain mucus on a cell layer that allows for macroscopic visualization of mucus distrubution. Our epithelium is grown in conventional...

31 January 2021 6,790 2 View

How to do immunofluorescence staining of the microtome sections of paraffin-embedded tissue ?

I plan to do immunofluorescence staining of microtome sections of paraffin-embedded metastatic tumor tissue with two or more than two antibodies with different emission spectra. Should I follow...

10 February 2021 3,113 3 View

Any suggestions to avoid the break of resin slices (Technovit 7100) on the microtome blade?

Hi everybody, I am beginning with the inclusion of biological samples (fish larvae) in resin (i.e. Technovit 7100). I am experimenting some troubles during the cut at the microtome (I would like...

16 November 2020 2,373 3 View

What would be the best histology technique to image teeth in confocal microscope using live/dead staining ?

Hello, To compare root canal irrigation techniques I am currently doing an experiment where I grow bacteria inside a tooth and then clean it using different irrigation. I then cut it into...

20 October 2020 1,927 4 View

Hello need help on Microtome. Anyone experiencing the blade cutting deeply FFPE tissue block after a few sections of the same tissue?

The blade is set at a clearance angle of 5 degrees at which it has been sectioning very well. In the past few days with no change of blade type or type of wax. The blade has been cutting in too...

20 October 2020 769 5 View

Suggestions for an easy experiment on an inverted fluorescence microscope (students lab course)?

09 September 2020 290 3 View

How often do you change a blade on a microtome?

Dear RG community, Histology related question: Can anyone advise on how many ffpe sections (estimated) can be cut using the same part of a microtome blade? The tissue I am sectioning is smooth,...

27 July 2020 1,297 3 View

Hi, I'd like to stain mitochondria with TMRM in situ, anyone has a protocol?

I would like to see by confocal microscopy the activity of the mitochondria potential membrane using TMRM. The problem is that I can't find a way to stain at TMRM in situ. I can process the...

04 March 2020 5,033 2 View

I have used hrp bound sencondary antibody developed with DAB+H2O2+PBS and i am not getting stain it seems like hematoxylin have stained only?

i have used protocol briefly paraffin embedded tissue 5um cut by microtome spread on water and take up on slide with overnight air drying hydration of tissue xylene 10min x2, Ethanol 100, 90, 70,...

06 February 2020 6,914 3 View

Is there a working protocol for microtomy of Vigna radiata root samples?

A simple and cost-effective protocol for sectioning of Vigna radiata root samples.

02 February 2020 786 1 View

How to begin slicing sections after a period in cryoperservation?

I have preserved large sections of sheep brain tissue in an ethylene glycol solution for later use. I'm hoping to take the sections out of solution and then slice on a microtome or cyrostat. How...

29 January 2020 1,884 3 View