Has anyone silver stained LR white embedded tissue?

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How to separate plasma from blood without centrifugation?

Hi Everyone, I am looking for methods that will all me to extract plasma from blood but WITHOUT centrifugation. Does anyone know any?

02 November 2021 1,756 5 View

Why do empty wells in a ThioT assay (plate is black) give a fluorescence signal?

Hi Everyone, We are optimizing a ThioT assay in our lab and we started noticing that wells that have nothing in them (the true negative) are giving off a fluorescence signal. As you can see...

19 August 2020 8,565 0 View

My His-Tagged Secondary Antibody for ELISA is causing background?

Hello Everyone, We have a secondary antibody that we biotinylated for detection in our ELISA system that is Strep-HRP and TMB based. However the his-tag is on the C-terminus and we did not get...

01 August 2020 4,404 7 View

Why is it not recommended to use expression E. coli for cloning?

Hi Everyone, We are having some difficulty doing E. coli cloning because our insert is toxic. We thought of using Rosetta E. coli (expression line) for cloning since it exherts tight control...

29 April 2020 6,705 7 View

Why do I keep getting a biofilm instead of single colonies?

Hi All, I have a pure plasmid with my gene of interest. The plasmid contains the a strep resistance gene for selection. I am using two different hosts: 1) Rosetta-gami™ 2(DE3)pLysS (host has...

16 April 2020 5,689 5 View

Is there a software (free or cheap) to manage animal breeding?

Hi Everyone, I have started an animal colony and already it is growing beyond something that is easily manageable. Previously (10 yrs ago and before my time) the lab looked into software to...

21 October 2019 3,675 5 View

Antibodies for alpha synuclein: Does anyone know a company to get a hybridoma?

Hi All, I need large (1-5 mg) amounts of alpha-synuclein antibody. It is not effective to purchase, but it is also not efficient or cost effective to make in-house (even if we had the...

21 August 2019 3,794 4 View

Determining whether peptide truncation is artifact or endogenous -TopDown Proteomics?

I am finding a number of peptides in brain that are truncated at either the N-terminus or C-terminus or both compared to the canonical sequence. What is the best way to determine if these are an...

26 September 2016 6,088 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How can I measure the thickness of thin film by Uv-Vis?

06 August 2024 7,810 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View