Hi Everyone,
We are optimizing a ThioT assay in our lab and we started noticing that wells that have nothing in them (the true negative) are giving off a fluorescence signal.
As you can see from the attached excel, we keep the edge of the plate blank because their is more variability in those areas. But also column 11 was left empty because we did not have enough sample to fill an entire plate, and we can see fluoresence values (first attachment is test 5).
Also, compared to initial pilot experiments we did, subsequent experiments showed a signal that when "over" very early in the assay (G10; 2nd attachment). The blank here is 10x higher than initial runs.
What is going on here?
Regards,
Norelle
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