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Questions related from Norelle C. Wildburger
Hello Everyone, We have a secondary antibody that we biotinylated for detection in our ELISA system that is Strep-HRP and TMB based. However the his-tag is on the C-terminus and we did not get...
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Hi Everyone, We are having some difficulty doing E. coli cloning because our insert is toxic. We thought of using Rosetta E. coli (expression line) for cloning since it exherts tight control...
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Hi All, I have a pure plasmid with my gene of interest. The plasmid contains the a strep resistance gene for selection. I am using two different hosts: 1) Rosetta-gami™ 2(DE3)pLysS (host has...
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Hi Everyone, I have started an animal colony and already it is growing beyond something that is easily manageable. Previously (10 yrs ago and before my time) the lab looked into software to...
22 October 2019 3,528 5 View
I am interested in using Bielschowsky's silver stain on brain tissue that has been embedded in LR White and sectioned 200-500 nm thin. Is there a protocol for this? Does silver stain work on both...
25 March 2018 4,153 2 View
I am finding a number of peptides in brain that are truncated at either the N-terminus or C-terminus or both compared to the canonical sequence. What is the best way to determine if these are an...
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