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Questions related from Norelle C. Wildburger
Hi Everyone, I am looking for methods that will all me to extract plasma from blood but WITHOUT centrifugation. Does anyone know any?
03 November 2021 1,716 5 View
Hi Everyone, We are optimizing a ThioT assay in our lab and we started noticing that wells that have nothing in them (the true negative) are giving off a fluorescence signal. As you can see...
20 August 2020 8,544 0 View
Hello Everyone, We have a secondary antibody that we biotinylated for detection in our ELISA system that is Strep-HRP and TMB based. However the his-tag is on the C-terminus and we did not get...
02 August 2020 4,363 7 View
Hi Everyone, We are having some difficulty doing E. coli cloning because our insert is toxic. We thought of using Rosetta E. coli (expression line) for cloning since it exherts tight control...
30 April 2020 6,662 7 View
Hi All, I have a pure plasmid with my gene of interest. The plasmid contains the a strep resistance gene for selection. I am using two different hosts: 1) Rosetta-gami™ 2(DE3)pLysS (host has...
17 April 2020 5,641 5 View
Hi Everyone, I have started an animal colony and already it is growing beyond something that is easily manageable. Previously (10 yrs ago and before my time) the lab looked into software to...
22 October 2019 3,548 5 View
Hi All, I need large (1-5 mg) amounts of alpha-synuclein antibody. It is not effective to purchase, but it is also not efficient or cost effective to make in-house (even if we had the...
22 August 2019 3,774 4 View
I am interested in using Bielschowsky's silver stain on brain tissue that has been embedded in LR White and sectioned 200-500 nm thin. Is there a protocol for this? Does silver stain work on both...
25 March 2018 4,165 2 View
I am finding a number of peptides in brain that are truncated at either the N-terminus or C-terminus or both compared to the canonical sequence. What is the best way to determine if these are an...
27 September 2016 6,060 1 View
